Dansyl- and rhodamine-based fluorescent sensors for detecting singlet oxygen and superoxide production in plants in vivo
نویسندگان
چکیده
Reactive oxygen species (ROS) play a key role in a variety of biotic and abiotic stress conditions, therefore their direct measurement in vivo is of special importance. In this study, we describe two dansyl-based ROS sensors, the singlet oxygen specific DanePy; HO1889NH, which is reactive to both singlet oxygen and superoxide radicals and a rhodaminebased singlet oxygen sensor, HO-2941. In order to characterise the potential use of these chemosensors in plant experiments in vivo, we characterise their ROS trapping ability, fluorescence emission spectra and localisation in spinach leaves. Acta Biol Szeged 46(3-4):149-150 (2002)
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